IWAN SASKIAWAN
LABORATORIUM MIKROBIOLOGI PANGAN BIDANG MIKROBIOLOGI PUSAT PENELITIAN BIOLOGI LEMBAGA ILMU PENGETAHUAN INDONESIA

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Journal : BERITA BIOLOGI

OPTIMASI PRODUKSI SERTA ANALISIS AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA SENYAWA EKSOPOLISAKARIDA DARI JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA CAIR [Optimization of Exopolysaccharide Production from Pleurotus ostreatus Growth on Liquid Medium and Analysis of Its Antioxidant and Antimicrobial Activity ] Saskiawan, Iwan; Munir, Misbahul; Achmadi, Suminar S
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2845.341 KB) | DOI: 10.14203/beritabiologi.v15i2.2299

Abstract

Pleurotus ostreatus is well known as an oyster mushroom that is very popular because of its high nutritional value and pharmaceutical component. The aim of this research was to study the antioxidant and antibacterial activity of exopolysaccharides from P. ostreatus grown on liquid medium.The P. ostreatus was grown on Mushroom Complete Medium (MCM) containing various types of Carbon (C) and Nitrogen (N) sources, i.e glucose, lactose, amylose and sucrose as a Carbon sources and yeast extract, polypeptone, NH4Cl, and NH4NO3 as a Nitrogen sources. The results showed that sucrose and yeast extract were the best source of Carbon and Nitrogen that produced 208 and 100 mg/L of exopolysaccharides.  The exopolysaccharide exhibited antimicrobial activity against Bacillus subtilis and Escherichiacoli. However, it did not have antimicrobial activity against Saccharomyces cerevisiae.  In addition, the exopolysaccharide indicated to have an antioxidant activity.
HIDROLISIS KITIN MELALUI FERMENTASI SEMI PADAT UNTUK PRODUKSI N-ASETILGLUKOSAMINA Saskiawan, Iwan; Handayani, Rini
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.249 KB) | DOI: 10.14203/beritabiologi.v10i6.1937

Abstract

N-acetyl-D-glucosamine (GlcNAc), the monomeric unit of polymer chitin has attracted much attention for their therapeutic activity in osteoarthritis.It is mainly produced by acid hydrolysis of chitin which affect the environmental problem because of its acidic wastes.Therefore, it is need to develop the new method for GlcNAc production. The aim of this experiment is to produce GlcNAc by mean of submerged fermentation of chitin. The preliminary study showed that fermentation of chitin by Aspergillus sp.501 produced higher GlcNAc than that of Saccharomyces sp. It was 1.39 ng/ml and 1.07 ng/ml. Then the Aspergillus sp. 501 was used in optimation of GlcNAc production. The effect of pH and nitrogen course such as bacto peptone, yeast extract, amonium sulfat and urea to GlcNAc production was examined. Then the product of GlcNAc was precipited by vacum evapotated and freeze dryed. The results showed that the highest of production GlcNAc of 2.228 ng/mL was obtained on pH 4 of medium solid state fermentation using urea as Nitrogen source at 10 days incubation.
SELEKSI DAN KARAKTERISASI MIKROBA LIGNOSELULOLITIK YANG DIISOLASI DARI LIMBAH SERBUK GERGAJI SEBAGAI MEDIA TANAM JAMUR TIRAM (PLEUROTUS OSTREATUS) Setiarto, R Haryo Bimo; Saskiawan, Iwan
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.514

Abstract

Spent Mushroom Substrate (SMS) is a composted growing medium that results from the mushroom growing process. The utilization of SMS for biofertilizer or soil conditioner would be an important point in green agriculture. The study revealed the lignocellulolytic activity from 20 isolates of Fungi and 13 isolates of Bacteria which were isolated from sawdust as a spent mushroom substrate of Pleurotus ostreatus. The selected microorganism then would be used as inocullant for the production of biofertilizer using SMS as a main material. The lignocellulolytic system consist of laccase, cellulase and xylanase activity was analyzed. The results shown that among 20 isolates of Fungi, the highest activity of laccase, cellulase, and xylanase was obtained from the isolates 2F1, 2F4 and 2F5. There was (6.153 U, 4.662 U, 3.791U) for laccase, (6.740, 3.711 U, 3.605 U) for cellulase and (6.870 U, 4.673 U, 3.773 U) for xylanase respectively for 2F1, 2F4 and 2F5. Furhtermore, the characterization of the highest lignocellulytic fungi was also conducted. The isolate 2F1 opimally grow in pH 5 at 40oC, isolate 2F4 in pH 9 at 30oC and isolate 2F5 in pH 5 at 30oC. The identification of isolated fungi are in the progress.